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  Cell culture is a cornerstone of biomedical research, drug development, and biomanufacturing—and cell culture media is the lifeline that determines cell health, proliferation, and functionality. At Yanbiotech, we provide researchers with a complete portfolio of high-quality cell culture solutions, including our flagship 9 core basal media formulations that cover virtually all mammalian cell culture needs. 1. Yanbiotech's 9 Core Basal Media Formulations Manufactured under strict ISO-certified conditions: Media  Key Features Typical Applications DMEM High/low glucose options HEK293, HeLa, fibroblasts MEM Eagle's basic formulation Adherent cell lines α-MEM With nucleosides Stem cells, osteoblasts RPMI 1640 Optimized for suspension Lymphocytes, hybridomas IMDM Rich nutrient profile Hematopoietic cells Ham's F-12 Complex nutrient mixture CHO cells, hepatocytes DMEM/F12 1:1 mixture Stem cells, 3D cultures McCoy's 5A Primary culture optimized Epithelial cells, biopsies M199 Contains multiple growth factors Endothelial cells   2.Technical Advantages Cell-Specific Optimization Guides available for all 9 media types Endotoxin-controlled: <0.1 EU/mL for sensitive applications Performance-matched to leading international standards Featured Product: 620405 (Nucleoside-supplemented α-MEM) Specifically formulated for mesenchymal stem cell expansion 3. Specialized Applications Advanced Research Solutions ✔ Organoid Media Kits (Matched matrix/media systems) ✔ CRISPR-Edited Cell Line Media (With optimized recovery supplements) ✔ Bioreactor-Grade Formulations (For scale-up studies) 4. Ordering & Support First-time user offer: Free technical consultation with purchase Contact our cell culture specialists: 🔬 Email: info@yanbioinstruments.com 🌐 Website: www.yanbioinstruments.com Follow the conversation: #CellCulture #Yanbiotech #ResearchSolutions #BiotechInnovation #LifeScience (Products for research use only. CE certified where applicable.)        

Cell culture experiments are a cornerstone of life science research, playing a vital role in drug screening, gene function studies, and disease mechanism exploration. To ensure accuracy and reproducibility, following a standardized experimental protocol is essential. This article provides a detailed guide to the basic steps of cell culture experiments, helping you achieve reliable results efficiently. 1. Pre-Experiment Preparation   1.1 Experimental DesignBefore starting, clearly define the objectives of your experiment and design a robust experimental plan. This includes determining experimental and control groups, selecting appropriate cell lines, and setting time points for data collection.1.2 Reagent and Instrument Preparation Cell Culture Medium: Choose a suitable medium (e.g., DMEM, RPMI-1640) based on the cell type, and supplement it with necessary serum and antibiotics. Trypsin: Used for cell detachment. PBS Buffer: For washing cells. Instruments: CO2 incubator, biosafety cabinet, centrifuge, inverted microscope, etc. 2. Cell Thawing and Culturing2.1 Cell Thawing Retrieve frozen cells from the liquid nitrogen tank and quickly thaw them in a 37°C water bath.   Transfer the cell suspension to a centrifuge tube containing pre-warmed medium, centrifuge, discard the supernatant, and resuspend the cells in fresh medium before seeding them into a culture flask. 2.2 Cell Passaging When cells reach 80%-90% confluency, remove the old medium and wash the cells with PBS. Add an appropriate amount of trypsin to detach the cells. Once cells round up, add medium to neutralize the trypsin. Centrifuge, discard the supernatant, and resuspend the cells in fresh medium for subculturing.   3. Experimental Treatment 3.1 Cell Seeding Seed cells into multi-well plates or culture dishes according to experimental needs, ensuring even cell distribution. Place the cells in a CO2 incubator and allow them to adhere and reach the desired density. 3.2 Drug Treatment or Transfection Drug Treatment: Add different concentrations of drugs to the cells based on the experimental design, including control and treatment groups. Cell Transfection: Introduce plasmids or siRNA into cells using lipofection or electroporation. 4. Cell Detection and Analysis 4.1 Cell Proliferation Assay Use CCK-8 or MTT assays to measure cell proliferation, and determine absorbance values using a microplate reader. 4.2 Apoptosis Detection Detect apoptosis rates using Annexin V/PI staining combined with flow cytometry. 4.3 Protein or Gene Expression Analysis   Extract total protein or RNA from cells and analyze target protein or gene expression levels via Western Blot or qPCR. 5. Data Organization and Analysis Organize experimental data and perform statistical analysis using software like GraphPad Prism. Create graphs and charts, and compile an experimental report summarizing the results and discussing their scientific significance. 6. Key Considerations Maintain strict aseptic techniques to avoid contamination. Regularly monitor cell health and growth conditions. By following this standardized cell culture protocol, you can efficiently complete experiments and obtain reliable data. Whether for basic research or drug development, meticulous experimental practices are key to success. If you need high-quality cell culture reagents or instruments, feel free to contact yanbiotech. We offer professional products and technical support to help you achieve your research goals! Document all experimental steps and conditions in detail to ensure reproducibility.  

Micropipettes are essential tools in any laboratory setting, enabling scientists to measure and transfer small volumes of liquids with incredible accuracy. Whether you’re conducting molecular biology experiments, chemical analyses, or clinical diagnostics, mastering the use of a Micropipette is crucial for achieving reliable results. In this guide, we’ll walk you through the basics of using this indispensable Lab Instrument and highlight why choosing the right brand matters.Step-by-Step Guide to Using a Micropipette Select the Right Micropipette: Micropipettes come in different volume ranges (e.g., 0.5-10 µL, 10-100 µL, 100-1000 µL). Choose the one that matches your required volume to ensure precision. Set the Volume: Adjust the volume using the adjustment dial. Make sure the display shows the correct measurement before proceeding. Attach a Tip: Use a sterile pipette tip that fits securely onto the Micropipette. Avoid touching the tip to prevent contamination. Aspirate the Liquid: Press the plunger to the first stop, immerse the tip into the liquid, and slowly release the plunger to draw the liquid into the tip. Dispense the Liquid: Place the tip into the target container and press the plunger to the first stop, then to the second stop to ensure all the liquid is expelled. Why Precision Matters A Micropipette is more than just a Lab Instrument; it's a gateway to accurate and reproducible science. Even a slight error in pipetting can lead to significant deviations in experimental outcomes. That’s why investing in a high-quality Micropipette is non-negotiable for any lab professional. Why Choose Yanbiotech Micropipette?When it comes to Micropipettes, yanbiotech stands out as a trusted brand in the scientific community. Our Micropipettes are engineered for precision, durability, and ease of use, ensuring consistent performance in even the most demanding lab environments. But what truly sets us apart is our commitment to customer satisfaction. From personalized product recommendations to responsive after-sales support, yanbiotech is dedicated to helping you achieve your research goals.In conclusion, mastering the use of a Micropipette is a fundamental skill for any lab professional. By choosing yanbiotech+Micropipette, you’re not just investing in a high-quality Lab Instrument—you’re partnering with a brand that prioritizes your success. Explore our range of Micropipettes today and experience the difference for yourself!

Selecting the ideal tissue homogenizer is critical for achieving consistent and reliable results across diverse laboratory workflows. Whether you’re preparing samples for a PCR experiment, optimizing protein extraction for a WB experiment, or processing complex biological tissues, the right homogenizer can significantly impact efficiency and data quality. Here’s a guide to help you make an informed decision. 1. Understand Your Sample Type and ThroughputThe first step in choosing a tissue homogenizer is evaluating your sample characteristics. Fragile tissues (e.g., plant leaves or cell cultures) may require gentle bead-based homogenization to preserve RNA integrity for sensitive PCR experiments. Conversely, tough fibrous tissues (e.g., muscle or tumors) might need high-shear rotor-stator systems for complete disruption. Additionally, consider throughput: high-volume labs often benefit from automated homogenizers, while smaller labs may prefer compact, manual models. 2. Match Homogenization Methods to Your Application(1) PCR Experiment: For nucleic acid isolation, prioritize homogenizers that minimize cross-contamination and heat generation. Bead mill systems or ultrasonic homogenizers are ideal for lysing cells without degrading DNA/RNA. (2) WB Experiment: Protein extraction demands efficient tissue disruption while maintaining protein stability. Mechanical homogenizers with adjustable speeds ensure optimal lysis buffer interaction, preserving epitopes for accurate Western blot results.(3) Multi-Application Labs: Versatile homogenizers with interchangeable accessories (e.g., blades, tubes) adapt to varying needs, from grinding tissues to emulsifying lysates. 3. Prioritize Reproducibility and Ease of UseConsistency is key in experiments like PCR and WB, where slight variations in sample prep can skew outcomes. Look for homogenizers with programmable settings, timers, and speed controls to standardize protocols. Ergonomic designs and easy-to-clean components also reduce user error and downtime—critical for busy labs processing dozens of samples daily. 4. Yanbiotech Tissue Homogenizers: Precision Meets Excellence When reliability matters, Yanbiotech tissue homogenizers stand out. Engineered for versatility, our homogenizers deliver unmatched performance across applications, from delicate PCR experiment prep to robust protein extraction for WB experiments. Key advantages include: (1) Advanced Technology:Patented cooling systems prevent heat-induced biomolecule degradation. (2) Customizable Configurations: Tailor your setup with specialized blades, tubes, or bead kits. (3) Unparalleled Support: Yanbiotech’s 24/7 technical team ensures seamless integration and troubleshooting, while our extended warranties guarantee long-term value. Choosing the right tissue homogenizer isn't just about equipment—it’s about investing in your lab’s future. With Yanbiotech, you gain more than a tool; you secure a partner committed to advancing your research through innovation, precision, and unwavering support. Explore our range today and elevate your PCR and WB experiments to new heights of accuracy.

Agarose gel electrophoresis is a conventional method for separating and identifying nucleic acids in molecular biology laboratories. Nucleic acids are amphoteric electrolytes with an isoelectric point of pH 2-2.5. In conventional electrophoresis buffers (pH about 8.5), nucleic acid molecules are negatively charged. The charge moves towards the positive pole in the electric field. Through electrophoresis technology, RNA molecules of different sizes and conformations can be separated, and by observing the number, size and shape of the bands, the integrity of the RNA molecules can be judged. In this process, the main function of the electrophoresis buffer is to maintain the pH and make the solution have a certain conductivity. TAE (Tris-acetic acid buffer) and TBE (TRIS-boric acid electrophoresis buffer) are commonly used buffers. What is the difference between them? TAE (Tris-acetate buffer) Advantage: ① For DNA fragments larger than 13kb, TAE buffer can achieve better separation results. ② In TAE buffer, the supercoiled state of DNA can better maintain its true relative molecular mass during electrophoresis. ③Suitable for recovering DNA fragments and subsequent enzyme digestion reactions, etc. shortcoming:   The buffer capacity is small, and TAE buffer may gradually lose its pH buffering capacity during long-term electrophoresis, resulting in changes in pH value. TBE (TRIS-boric acid electrophoresis buffer) Advantage: ① The buffering capacity is relatively stronger, the pH value can be kept constant even during long-term electrophoresis, and it is not easy to cause overheating in the electrophoresis tank. ② Suitable for electrophoretic separation of smaller fragments, such as fragments less than 1kb. Its high resolution makes it easier to distinguish molecules of different sizes, thereby increasing the accuracy of experiments. shortcoming: The boric acid component contained may affect the recovery efficiency of RNA and DNA and subsequent enzymatic reaction experiments.    

Hey, friends! Today I’m going to take you into a super cool field – cell culture! Cell culture is actually a technology that artificially controls the cell growth environment in the laboratory. Simply put, it is to create an ideal living environment for cells, allowing them to grow, divide and spread freely. Imagine that this is like building a small "home" for the cells, allowing them to express their abilities and potential here! Cell culture applications are literally everywhere! In biomedicine, cell culture helps us study the mysteries of disease, screen new drugs, and evaluate drug efficacy. Did you know? These little cell culture "helpers" have brought many anti-cancer drugs, vaccines and other medical advances to mankind! Not only that, in the field of biotechnology, cell culture is also indispensable. Scientists can use cell culture technology to produce important proteins, hormones and enzymes, and even perform genetic engineering and biosynthesis. These technologies not only have great potential in the medical field, but also play an important role in the research and development of many areas of high social concern, such as biofuels and renewable energy. ?? Of course, cell culture is a complex and sophisticated technology that requires specialized operations and deep expertise. Scientists must not only select suitable cell lines, but also provide appropriate culture media and growth conditions, and monitor and control the growth status of the cells. Here are some basic steps and key points for cell culture: Select cells: First, you need to select the cell type you want to culture. Depending on the purpose and needs of the research, different types of cells can be selected, such as tumor cells, primary cells, or cell lines transfected with specific genes. Prepare culture medium: Make culture medium appropriate for the cell type of choice. Culture medium is a liquid solution containing nutrients, growth factors, and supplements that provide the nutrients and environmental conditions needed for cell culture. Cell dissociation and passaging: Dissociate cultured cells from culture vessels (such as Petri dishes or flasks) and redisperse them into new culture vessels. This process, called cell passaging, keeps cells growing and multiplying. Control culture conditions: Control culture conditions, including temperature, humidity, CO2 concentration and pH value of the culture medium. These conditions help provide a suitable cellular environment and promote healthy cell growth. Contamination control: Sterile conditions must be maintained during cell culture to prevent contamination by bacteria, fungi and other cells. Adopting strict handling and sterilization procedures is key to ensuring culture purity. Observation and experimental operations: Regularly observe the morphology, growth and cell density of cultured cells. According to specific experimental needs, cell processing, drug treatment, gene transfection and other operations are performed. Cell culture is not only a technology, but also an art. Every successful cultivation is a reverence for life and the pursuit of science. I hope this article gives you a deeper understanding of cell culture! ??  

1. Extraction of total tissue protein: 2.1 Wash the tissue 1-2 times with pre-cooled PBS, cut into small pieces and place in a grinding tube, add 3pcs, 3mm grinding beads, and add 10 times the tissue volume of lysis solution (for example: 100mg of tissue, add 1000ul of lysis solution liquid), set the grinding program for tissue grinding; 2.2 Take out the grinding tube after grinding and place it on ice or in fourth-degree lysis solution for 30 minutes; 2.3 Centrifuge at 12000 rpm, 4°C for 10 minutes, collect the supernatant, which is the total protein solution. 2. Protein concentration determination (optional): Determine the protein concentration as needed, take the undenatured protein solution, and use the BCA protein concentration determination kit to measure the protein concentration. For specific methods, refer to the kit instructions; 3. Protein denaturation:Add 5* reduced protein loading buffer to the protein solution at a ratio of 4:1, denature it in a metal bath at 95°C for 10 minutes, and store it in a -20°C or -80° refrigerator for later use; 4. Electrophoresis 4.1 Clean the glass plate; 4.2 Gel preparation and sample loading; 4.2.1 ①. First, move the clamping plates on both sides to the bottom, completely open the glands on both sides, insert the concave glass and flat glass from the top diagonally and place them to the bottom. The upper part of the glass is stuck in the slots on both sides; ②. Turn up the glands on both sides, pinch the left part of the gland with your hands at the same time, pull the left clamping plate upward, and lock it to the top; then pinch the right side of the gland at the same time, pull the right clamping plate upward, and lock it. to the highest; ③. After confirming that the electrophoresis glass is clamped and aligned, unscrew the knobs on both sides of the gel making base, then place the electrophoresis bracket into the middle of the gel making base and clamp it, then press the main body bracket with your hands and tighten the knobs on both sides until Rotate to the limit. 4.2.2 Select gel making kits of different concentrations according to experimental requirements, mix solutions A and B in equal proportions, and prepare lower gel solution and upper gel solution respectively. For glass plates of different specifications and thicknesses, the volumes of the upper glue and lower glue solutions can be adjusted in equal proportions. Taking the commonly used 8.3 cm × 7.3 cm gel plate (single piece) as an example, the recommended preparation system is as follows:; Formulation group Formulation 0.75 mm Glass Plate 1.0 mm Glass Plate 1.5 mm Glass Plate Lower glue solution 10% Lower glue solution A 2 mL 2.5 mL 4 mL 10% Lower glue solution B 2 mL 2.5 mL 4 mL AP 24 μL 30 μL 48 μL Upper glue solution Upper glue solution A 1 mL 1 mL 1.5 mL Upper glue solution B(Red Color) 1 mL 1 mL 1.5 mL AP 12 μL 12 μL 18 μL 4.2.3 After assembling the glue maker, first add the prepared lower glue solution, and then use pure water or ethanol to seal the lower glue surface. After the lower glue has fully solidified (about 10-15 minutes), discard the water or ethanol, and use filter paper to absorb the remaining liquid, then add the prepared upper glue solution, insert the comb, and wait for it to solidify (about 10-15 minutes) before use; 4.2.4 Remove the main body of the gel maker, carefully pull out the comb, and prepare to start electrophoresis; 5. After placing the main body of the gel maker into the electrophoresis tank, fill the inside with electrophoresis buffer and add 1/3 with the outside. Use a constant voltage of 200V for 30 minutes until the bromophenol blue is approximately 1cm away from the bottom. The electrophoresis is terminated and the electrophoresis is ready for transfer. 6. Transfer film 6.1 Prepare 6 pieces of 7×9cm transfer filter paper (thin) and 5×8cm PVDF membrane. The PVDF membrane must be activated with ethanol for 2 minutes before use; 6.2 Put the transfer clip, two sponges, filter paper and activated PVDF membrane in the container with transfer solution; 6.3 Spread the transfer folder, with red on the left and black on the right. Add a sponge and three pieces of filter paper to each side; 6.4 Carefully peel off the separation glue and place it on the filter paper (the glue is placed on the side of the black transfer clip). Use the transfer liquid to rinse the bubbles on the glue. Slowly stick the PVDF film to the glue. Be careful not to have any bubbles. Then stick the transfer film in turn. Membrane filter paper, transfer sponge; 6.5 Transfer conditions (wet transfer): Constant current, 300mA for half an hour. 7. Immune response 7.1 Place the transferred membrane into an incubation box containing TBST, rinse it quickly, then add 5ml of 5% skimmed milk, place it on a decolorizing shaker, and block at room temperature for 30 minutes; 7.2 According to the antibody instructions, dilute the primary antibody. After configuration, pour out the blocking solution in the incubation box, add the prepared primary antibody, and incubate at 4°C on a shaker overnight (shake the shaker slowly); 7.3 Recover the primary antibody, rinse the membrane quickly with TBST three times, then add TBST, place it on a destaining shaker for rapid elution, wash three times for 5 minutes each time; 7.4 Dilute the secondary antibody with TBST at a ratio of 1:5000, then add it to the incubation box, place it on a shaker and shake slowly, and incubate at room temperature for 30 minutes; 7.5 Rinse the membrane quickly with TBST three times, then add TBST, place it on a destaining shaker for rapid elution, wash three times for 5 minutes each time. 8. ChemiluminescenceMix ECL A and B solutions in a ratio of 1:1 and set aside. Take out the eluted PVDF membrane and place it on absorbent paper. Slightly absorb the liquid on the membrane and put the membrane into the mixed ECL. In the luminescent liquid, let the liquid completely immerse the membrane. After the reaction for 1 minute, take out the membrane and place it on the chemiluminescence instrument tray. Start chemiluminescence according to the preset program. After the exposure is completed, save the original image in TIFF format. 9. WB results and analysis 

Colleagues who sell pipettes often receive inquiries from recommended customers, "Can you give me a set of pipettes? They can be used in all measuring ranges, like a set of four." At the request of customers, pipettes with four measuring ranges of 0.5-10uL, 5-50uL, 10-100uL, and 100-1000uL are generally recommended to customers. On the surface, it seems that what the customer buys covers almost the full range of micropipetting, and it seems that everything is done perfectly, but this is not the case! After many years of pipette after-sales work, we have collected feedback from customers on problems such as inaccurate pipetting and difficulty in use, which often stem from the initial set selection.Let's take the most common example: Customers often give the most feedback in pipetting when using a 10uL pipette to transfer liquids within 1uL. Theoretically speaking, a pipette with a measuring range of 0.5-10uL has no problem when operating liquids within 0.5-1uL. Why do customers often report the most problems at this measurement range? Below 1uL is an ultra-micro liquid operation. The amount of liquid is almost invisible to the naked eye. The basic skills of liquid operation are very high, such as: rinsing, insertion depth and other technical details.For example: technical details such as rinsing, insertion liquid level depth, etc. In addition, the quality of the suction head also has a great impact on this micro-volume operation. Some suction tips of poor quality will have residual plastic burrs on the tip end of the suction head. Such poor quality tips have almost fatal effects on micropipetting. When using tips of normal quality, most operators choose to use pipettes with a range of 0.1-2uL. For liquid transfer operations below 1uL, the pipetting effect is significantly better than pipettes with a range of 0.5-10uL. Below we take the Yanbio pipette as an example to illustrate:The general range of ordinary precision adjustable pipettes is between 0.1uL and 10mL. The different ranges are shown in the table below: Item Name Cat.No. Nominal Volume Volume Range Increment Single Channel Pipette YB-P2.5 2.5 μL 0.1-2.5 μL 0.05 μL YB-P10 10 μL 1-10 μL 0.1 μL YB-P20 20 μL 2-20 μL 0.1 μL YB-P100 100 μL 10-100 μL 1 μL YB-P200 200 μL 20-200 μL 1 μL YB-P1000 1000 μL 100-1000 μL 5 μL YB-P5000 5000 μL 1000-5000 μL 50 μL YB-P10000 10 mL 1-10 mL 100 μL 8-Channel Pipette YB-PM10 10 μL 1-10 μL 0.1 μL YB-PM100 100 μL 10-100 μL 1 μL YB-PM300 300 μL 50-300 μL 1 μL YB-PM1000 1000 μL 100-1000 μL 5 μL   Different models of pipettes have different ranges. When we choose a pipette, a certain range will often appear, and several pipettes of different models can suffice. How to choose the pipette with the most suitable volume. Example: A pipette is required, and the volume often pipetted is 20uL. We have four different types of 2-20uL (model YB-P20) / 5-50uL (model YB-P50) / 10-100uL (model YB-P100) / 20-200uL (model YB-P200) Liquid dispensers are available. Which pipette has the smallest error at 20uL? According to our daily routine of selecting products, we believe that it would be most appropriate if the target volume is in the middle range, and we will empirically choose 5-50uL (model YB-P50). Is this the case? Let's take a look at the data results:Let's use these four types of pipettes to do an error analysis: Cat. No.: Volume Error at 20uL YB-P20 20-20uL ±0.2uL YB-P50 5-50uL ±0.5uL YB-P100 10-100uL ±0.6uL YB-P200 20-200uL ±1.0uL It can be seen that the 2-20uL pipette is the most suitable in terms of accuracy and precision, and the target volume of 20uL is the most suitable.