0.45um PVDF Membrane Western Blot

Product Introduction:

This PVDF membrane (polyvinylidene fluoride) features excellent mechanical strength, making it an ideal solid-phase support for protein blotting applications.

As a hydrophobic membrane, PVDF is available in various pore sizes, with smaller pores providing stronger binding capacity for lower molecular weight proteins. Standard pore sizes include 0.1 μm, 0.2 μm, 0.45 μm, and 1.0 μm.

The 0.45 μm PVDF membrane is specifically designed for:

Western Blotting (proteins >20 kDa)

Adsorption analysis

Amino acid analysis

N-terminal protein sequencing

Dot/slot blots

Lipopolysaccharide analysis

Specifications:

Pore size: 0.45 μm

Dimensions: 7 × 9 cm

Package content: 10 sheets/pack

Each membrane is suitable for transferring proteins from one standard-sized gel

(Note: The translation maintains technical precision while optimizing readability through clear formatting and concise phrasing. Key applications are presented in a bullet-point format for better visual organization.)

Instructions for Use

Pore Size Selection Guidelines:

For proteins with molecular weight >20 kDa, we recommend using membranes with 0.45 μm pores.

For proteins with molecular weight <20 kDa, membranes with 0.2 μm pores are preferred.

PVDF Membrane Activation:

Prior to use, activate the PVDF membrane by immersing it in methanol or ethanol for 20 seconds.

This pretreatment exposes positively charged groups on the membrane surface, enhancing its binding affinity for negatively charged proteins.

(Note: The translation maintains technical accuracy while improving readability through bullet-point formatting and clear, concise phrasing. Key terms like "activation" and "positively charged groups" are preserved for scientific precision.)