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100× Cocktail Protease Inhibitor without EDTA

100× Cocktail Protease Inhibitor without EDTA

Can be used in cell or tissue extracts to enhance protein stability

 

  • Cat. No. :

    Y20514-1ML
  • Brand :

    Yanbiotech
  • Product Origin :

    China
  • Volume :

    1mL
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  • Product Details

Product Information:

Component  Concentration Target
AEBSF 104 mM Serine proteases
Aprotinin 80 μM Serine proteases
Bestatin 5 mM Aminopeptidases
E-64 1.5 mM Cysteine proteases
Leupeptin 2 mM Serine and cysteine proteases
Pepstatin A 1.5 mM Aspartic proteases

 

A mixture of 6 broad-spectrum protease inhibitors to protect protein integrity.

The production and degradation of endogenous proteins are generally maintained in a state of equilibrium, so protein content within cells is stable under stable environmental conditions. However, in in vitro studies, protein synthesis ceases while degradation is greatly enhanced, thus affecting protein yield. Therefore, we recommend using a mixture of inhibitors composed of various small molecules to inhibit protease function and prevent protein degradation.


Storage Conditions:

-20ºC ,12 months.


Application:

Protein purification, Western blotting, Immunoprecipitation (Co-IP), Protein pull-down assays, Immunofluorescence, Immunohistochemistry, Kinase assays, etc.


Instructions for Use:

Thaw the DMSO stock solution at room temperature. Add the inhibitor mixture to your experimental system (e.g., cell lysate or tissue extract) at a 1:100 ratio.

 

 

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