α-Minimum Essential Medium Nucleoside Stem Cell Culture Media

Product Introduction

Our α-Minimum Essential Medium (α-MEM) is a high-performance, serum-free formulation designed to accelerate the growth and maintenance of primary cells and stem cells. Enriched with essential nucleosides and GlutaPlus™, this medium provides optimal nutrient balance to support faster cell proliferation, improved viability, and sustained undifferentiated states.

This modified MEM formulation is supplemented with Non-Essential Amino Acids (NEAA), L-alanyl-L-glutamine (GlutaPlus™), and HEPES buffer to enhance cell growth and medium stability. It is suitable for a wide range of mammalian cells, including primary cultures and fastidious cell lines.

Product Specification

PRODUCT HIGHLIGHT

✔ 2 mM L-alanyl-L-glutamine - Reduced concentration for glutamine-sensitive applications

✔ Complete nucleoside/deoxynucleoside supplement

✔ 1 mM sodium pyruvate - Enhanced energy metabolism support

✔ Optimized for:

• Primary cell cultures
• Metabolic studies
• Long-term maintenance

KEY APPLICATION

• Stem cell maintenance (lower glutamine reduces differentiation stress)
• Neuron/glia co-cultures (sensitive to ammonia buildup)
• Replicative senescence studies

QUALITY CONTROL

• Endotoxin: <0.1 EU/mL
• Sterility: 0.1 μm filtered
• PH Stability: 7.0-7.4 (5% CO₂)

STORAGE:

• Temp: 2–8°C
• Shelf Life: 12 months