MEM Stem Cell Culture Medium with NEAA & GlutaPlus

Product Introduction

This modified MEM formulation is supplemented with Non-Essential Amino Acids (NEAA), L-alanyl-L-glutamine (GlutaPlus™), and HEPES buffer to enhance cell growth and medium stability. It is suitable for a wide range of mammalian cells, including primary cultures and fastidious cell lines.

Product Specification

MEM with NEAA, GlutaPlus™ (1.87 mM), HEPES (25 mM), and 5.5 mM D-Glucose

PRODUCT DESCRIPTION

A modified Minimum Essential Medium (MEM) formulation containing:

• 1.87 mM L-alanyl-L-glutamine (GlutaPlus™) for stable glutamine delivery
• 25 mM HEPES for enhanced pH buffering
• 5.5 mM D-glucose (1.0 g/L)
• 1× Non-Essential Amino Acids (NEAA)

 PHYSICAL PROPERTIES

• PH: 7.2–7.4 (at 37°C, 5% CO₂)
• Osmolarity: 290–310 mOsm/kg
• Color: Red (pH indicator)
• Sterility: 0.1 μm filtered
• Phenol red pH indicator

RECOMMENDED USE

• Target Applications
• Primary cell culture (neurons, epithelial cells)
• Long-term experiments (>72 hours)

Supplementation Guidelines

• Serum: 5–10% FBS (recommended)
• Antibiotics: Optional (e.g., 1% penicillin-streptomycin)

Storage Temperature:

• Temp: 2–8°C
• Shelf Life: 12 months